Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Quorum-quenching enzyme Est816 assisted antibiotics against periodontitis induced by Aggregatibacter actinomycetemcomitans in rats

doi: 10.3389/fcimb.2024.1368684

Figure Lengend Snippet: Minimum inhibitory concentration (MIC) results for A. actinomycetemcomitans of (A) Minocycline, (B) Metronidazole and (C) Amoxicillin. The antibiotic concentrations ranged from 0.125 to 128 μg/mL. For the inactivated enzyme group, the enzyme was inactivated by heating. The error bars in each panel represent the SD of triplicates.

Article Snippet: 50 μL of the bacterial suspension (1× 10 6 CFU/mL) (OD 600 = 0.1) was added to equal volume of metronidazole (MTZ), amoxicillin (AMX), and minocycline (MINO) on the 96-well plate (MedChemExpress, NJ, U.S.A.) ( ).

Techniques: Concentration Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Quorum-quenching enzyme Est816 assisted antibiotics against periodontitis induced by Aggregatibacter actinomycetemcomitans in rats

doi: 10.3389/fcimb.2024.1368684

Figure Lengend Snippet: Morphology and biofilm formation of A . actinomycetemcomitans . (A) SEM revealed the effect of 12 U/mL lactonase Est816 with and without antibiotics on A . actinomycetemcomitans biofilm (original magnification × 1,000 and × 5,000). (B) Biofilm inhibition rate of minocycline (MINO), metronidazole (MTX) and amoxicillin (AMX) alone and in combination with enzyme Est816. (C) Biofilm eradication rate of MINO, MTZ, and AMX alone and in combination with enzyme Est816. (Compare within groups, ** P <0.01, *** P <0.001).

Article Snippet: 50 μL of the bacterial suspension (1× 10 6 CFU/mL) (OD 600 = 0.1) was added to equal volume of metronidazole (MTZ), amoxicillin (AMX), and minocycline (MINO) on the 96-well plate (MedChemExpress, NJ, U.S.A.) ( ).

Techniques: Inhibition

Journal: Antimicrobial Agents and Chemotherapy

Article Title: First Penicillin-Binding Protein Occupancy Patterns of β-Lactams and β-Lactamase Inhibitors in Klebsiella pneumoniae

doi: 10.1128/AAC.00282-18

Figure Lengend Snippet: Principal component analysis of the log-transformed PBP IC50 data for our 13 tested compounds in two K. pneumoniae strains. The plot shows the clustering of compounds according to their positions on the first and second eigenvector. These two vectors explained 86.5% of the total variance. Compounds were grouped into two general clusters. The first cluster contained β-lactams that primarily targeted PBP3 but differed in their secondary targets. The second cluster was comprised of compounds that primarily targeted PBP2 or both PBPs 2 and 4. Of note, among compounds in the second cluster, the carbapenems, amoxicillin, and mecillinam had substantially lower IC50 for their primary targets than the β-lactamase inhibitors. Symbols for β-lactamase inhibitors are smaller due to their much higher IC50 for their primary PBP target relative to the primary PBP target IC50 of the β-lactams.

Article Snippet: Biapenem was purchased from TRC Canada (Toronto, Ontario, Canada); doripenem and avibactam were from MedChem Express (Monmouth Junction, NJ); imipenem and meropenem were from AK Scientific (Union City, CA); sulbactam was from TCI America (Portland, OR); tazobactam, amoxicillin, piperacillin, aztreonam, cefepime, and cefsulodin were from Chem-Impex International, Inc. (Wood Dale, IL); and mecillinam was from Molekula (Newcastle Upon Tyne, United Kingdom).

Techniques: Transformation Assay

Journal: ACS Omega

Article Title: Synthesis and Characterization of Novel Core–Shell ZnO@SiO 2 Nanoparticles and Application in Antibiotic and Bacteria Removal

doi: 10.1021/acsomega.2c04226

Figure Lengend Snippet: Parameters for Amoxicillin (AMX) Adsorption Isotherms on ZnO@SiO 2 at Different Ionic Strengths Fitted by Langmuir and Freundlich Models

Article Snippet: Amoxicillin trihydrate (AMX), with purity >98% for high-performance liquid chromatography (HPLC), was delivered from AK Scientific (USA).

Techniques: Adsorption, Concentration Assay

Journal: ACS Omega

Article Title: Synthesis and Characterization of Novel Core–Shell ZnO@SiO 2 Nanoparticles and Application in Antibiotic and Bacteria Removal

doi: 10.1021/acsomega.2c04226

Figure Lengend Snippet: Adsorption Capacity and the Removal Efficiency of Amoxicillin (AMX) Using ZnO@SiO 2 Based on Rice Husk and Other Absorbents a

Article Snippet: Amoxicillin trihydrate (AMX), with purity >98% for high-performance liquid chromatography (HPLC), was delivered from AK Scientific (USA).

Techniques: Adsorption, Modification